Hall W W, Liu C R, Schneewind O, Takahashi H, Kaplan M H, Röupe G, Vahlne A
Division of Infectious Disease, North Shore University Hospital-Cornell University Medical College, Manhasset, NY 11030.
Science. 1991 Jul 19;253(5017):317-20. doi: 10.1126/science.1857968.
Mycosis fungoides, a rare form of cutaneous T cell leukemia/lymphoma, is suspected of having a viral etiology on the basis of certain similarities to adult T cell leukemia, which is associated with human T cell leukemia/lymphoma virus type I (HTLV-I) infection. Cell lines were established from peripheral blood mononuclear cells (PBMC) of an HTLV-I-seronegative patient with mycosis fungoides. DNA hybridization analysis revealed the presence of HTLV-I-related sequences with unusual restriction endonuclease sites. Sequence analysis of subcloned fragments demonstrated the presence of a monoclonally integrated provirus with a 5.5-kilobase deletion involving large regions of gag and env and all of pol. Additional evidence for the presence of deleted proviruses was found by polymerase chain reaction (PCR) amplification of DNA from cutaneous lesions of five other HTLV-I-seronegative patients. The findings suggest that HTLV-I infection may be involved in the etiology of at least certain cases of mycosis fungoides.
蕈样肉芽肿是一种罕见的皮肤T细胞白血病/淋巴瘤，基于其与成人T细胞白血病的某些相似性，怀疑其具有病毒病因，成人T细胞白血病与人类T细胞白血病/淋巴瘤病毒I型（HTLV-I）感染有关。从一名患有蕈样肉芽肿的HTLV-I血清阴性患者的外周血单个核细胞（PBMC）中建立了细胞系。DNA杂交分析显示存在具有异常限制性内切酶位点的HTLV-I相关序列。亚克隆片段的序列分析表明存在一种单克隆整合的前病毒，其有一个5.5千碱基的缺失，涉及gag和env的大片区域以及全部pol。通过对其他五名HTLV-I血清阴性患者皮肤病变的DNA进行聚合酶链反应（PCR）扩增，发现了缺失前病毒存在的更多证据。这些发现表明，HTLV-I感染可能至少在某些蕈样肉芽肿病例的病因中起作用。
