Giovannini E, Principato G B, Ambrosini M V, Grassi G, Dell'Agata M
J Pharmacol Exp Ther. 1978 Aug;206(2):398-404.
The authors studied the variations of some blood parameters and urine composition during the first hours after CoCl2 treatment in rats and rabbits. These showed an increase in hematocrit with a decrease in plasma volume and, at the same time, a decrease in total proteinemia as well as in the albumin (rabbits) and gamma-globulin fractions. On the basis of observations carried out in rabbits, these phenomena are not seen if the cobalt is complexed with cysteine before treatment; moreover, they are at least partly reversed by cysteine administration soon after cobalt. Plasma Na+ and K+ concentrations do not show significant changes in CoCl2-treated rabbits; on the contrary, the excretion of such electrolytes, as well as urinary protein content, decreases. In addition, the urine of CoCl2-treated rabbits contains at least two pigmented substances with different molecular weights, which are separable by dialysis and DEAE-cellulose chromatography and both contain cobalt in different percentages. The authors believe that CoCl2 treatment leads to an early and profound increase in vascular permeability with depletion of plasmatic material in the tissues and hemoconcentration; moreover, the above stated phenomena seem to be correlated with the forming of cobalt complexes.
作者研究了氯化钴处理后大鼠和家兔最初几小时内一些血液参数和尿液成分的变化。这些变化表现为红细胞压积升高，血浆量减少，同时总蛋白血症以及白蛋白（家兔）和γ-球蛋白组分减少。根据在家兔身上进行的观察，如果在处理前将钴与半胱氨酸络合，则不会出现这些现象；此外，在钴处理后不久给予半胱氨酸，这些现象至少会部分逆转。在氯化钴处理的家兔中，血浆钠和钾浓度没有显著变化；相反，这些电解质的排泄以及尿蛋白含量减少。此外，氯化钴处理的家兔尿液中至少含有两种分子量不同的有色物质，可通过透析和二乙氨基乙基纤维素色谱法分离，且两者含钴百分比不同。作者认为，氯化钴处理会导致血管通透性早期显著增加，组织中血浆物质耗竭和血液浓缩；此外，上述现象似乎与钴络合物的形成有关。
