Gosden R G, Boulton M I, Grant K, Webb R
Department of Physiology, University Medical School, Edinburgh, UK.
J Reprod Fertil. 1994 Aug;101(3):619-23. doi: 10.1530/jrf.0.1010619.
Cortical slices of either cat or sheep ovaries were grafted under the renal capsules of ovariectomized SCID mice. The grafts became vascularized and were still surviving with large follicles present at autopsy up to nine months later. As developing follicles undergo atresia during the period of ischaemia after ovarian grafting, those found in long-term grafts at autopsy had presumably started to grow from the primordial stage after transplantation. Some follicles had reached a diameter of 3 mm with a normal antrum and appeared to be cytologically normal, but the latent period for the emergence of antral follicles was shorter in cat compared with sheep grafts. Oestradiol production from grafts, as indicated by vaginal cornification and plasma measurements collected at autopsy, was not constant and circulating concentrations varied among animals, and were sometimes far in excess of the normal physiological range of the host. The vaginal smears never presented cytological patterns like those of the normal mouse oestrous cycle, and ovulation had not occurred in any of the grafts. These results demonstrate that ovarian xenografts in SCID mice can serve as experimental models for investigating follicle development in species in which follicle growth in vitro and studies of the parent animal are impracticable.
将猫或绵羊卵巢的皮质切片移植到卵巢切除的SCID小鼠的肾被膜下。移植物形成了血管，在长达九个月后的尸检中仍存活，有大量卵泡。由于卵巢移植后缺血期间发育中的卵泡会发生闭锁，因此尸检时在长期移植物中发现的卵泡可能是移植后从原始阶段开始生长的。一些卵泡直径达到3毫米，有正常的卵泡腔，细胞学上似乎正常，但与绵羊移植物相比，猫移植物中窦状卵泡出现的潜伏期较短。根据尸检时收集的阴道角化和血浆测量结果表明，移植物产生的雌二醇并不恒定，不同动物的循环浓度有所不同，有时远远超过宿主的正常生理范围。阴道涂片从未呈现出正常小鼠发情周期那样的细胞学模式，任何移植物中都未发生排卵。这些结果表明，SCID小鼠中的卵巢异种移植物可作为实验模型，用于研究在体外卵泡生长和对亲本动物进行研究不可行的物种中的卵泡发育。
