Bosák Juraj, Micenková Lenka, Vrba Martin, Ševčíková Alena, Dědičová Daniela, Garzetti Debora, Šmajs David
Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
PLoS One. 2013 Dec 10;8(12):e81829. doi: 10.1371/journal.pone.0081829. eCollection 2013.
Colicin FY is a plasmid encoded toxin that recognizes a yersinia-specific outer membrane protein (YiuR) as a receptor molecule. We have previously shown that the activity spectrum of colicin FY comprises strains of the genus Yersinia. In this study, we analyzed the activity of colicin FY against 110 Yersinia enterocolitica isolates differing in geographical origin and source. All isolates were characterized through analysis of 16S rRNA genes, serotyping, biotyping, restriction profiling of genomic DNA, detection of virulence markers and susceptibility to antibiotics. This confirmed the broad variability of the collection, in which all 110 Y. enterocolitica isolates, representing 77 various strains, were inhibited by colicin FY. Although isolates showed variable levels of susceptibility to colicin FY, it was not associated with any strain characteristic. The universal susceptibility of Y. enterocolitica strains to colicin FY together with the absence of activity towards strains outside the Yersinia genus suggests potential therapeutic applications for colicin FY.
大肠杆菌素FY是一种由质粒编码的毒素，它识别一种耶尔森氏菌特异性外膜蛋白（YiuR）作为受体分子。我们之前已经表明，大肠杆菌素FY的活性谱包括耶尔森氏菌属的菌株。在本研究中，我们分析了大肠杆菌素FY对110株不同地理来源和来源的小肠结肠炎耶尔森氏菌分离株的活性。所有分离株均通过16S rRNA基因分析、血清分型、生物分型、基因组DNA限制性图谱分析、毒力标记检测和抗生素敏感性检测进行了表征。这证实了该集合的广泛变异性，其中所有110株小肠结肠炎耶尔森氏菌分离株，代表77个不同菌株，均被大肠杆菌素FY抑制。尽管分离株对大肠杆菌素FY的敏感性水平各不相同，但这与任何菌株特征均无关联。小肠结肠炎耶尔森氏菌菌株对大肠杆菌素FY的普遍敏感性以及对耶尔森氏菌属以外菌株无活性表明大肠杆菌素FY具有潜在的治疗应用价值。
