Asplund K, Johansson T, Siitonen A
National Public Health Institute, Department of Enteric Pathogens, Helsinki, Finland.
Epidemiol Infect. 1998 Dec;121(3):579-86. doi: 10.1017/s0950268898001629.
One hundred and six Yersinia enterocolitica serogroup O:3, biotype 4 isolated from human and porcine samples in 1984 and in the years 1993 5 were examined by pulsed-field gel electrophoresis (PFGE). The genomic profiles produced by the enzymes NotI and XbaI were studied. Sixteen (A-P) and 8 (1-8) different pulsotypes were obtained, respectively. By combining the pulsotypes produced by both NotI and XbaI 24 different types were distinguished. The two major types, designated as A1 and B1, comprised 36% of all strains tested. The proportions of pulsotypes A1 and B1 were, 35.9 and 25.6%, respectively, among strains isolated in 1984. The corresponding figures among the strains isolated in 1993-5 were 35.8 and 41.8%. Nine pulsotypes were found only in 1984 and nine only in 1993-5. The proportions of the major pulsotypes, A1 and B1, in human isolates were 42.9 and 35.7% and in porcine isolates 22.2 and 36.1% respectively. Six types were found among both human and porcine isolates, 8 only among human strains and 10 only among porcine strains.
对1984年以及1993年至1995年期间从人和猪的样本中分离出的106株小肠结肠炎耶尔森菌O:3血清型、生物4型菌株进行了脉冲场凝胶电泳（PFGE）检测。研究了由NotI和XbaI酶产生的基因组图谱。分别获得了16种（A - P）和8种（1 - 8）不同的脉冲型。通过合并NotI和XbaI产生的脉冲型，区分出24种不同类型。两种主要类型，命名为A1和B1，占所有测试菌株的36%。在1984年分离的菌株中，脉冲型A1和B1的比例分别为35.9%和25.6%。在1993年至1995年分离的菌株中，相应的数字分别为35.8%和41.8%。9种脉冲型仅在1984年发现，9种仅在1993年至1995年发现。主要脉冲型A1和B1在人分离株中的比例分别为42.9%和35.7%，在猪分离株中的比例分别为22.2%和36.1%。在人和猪的分离株中都发现了6种类型，仅在人菌株中发现了8种，仅在猪菌株中发现了10种。
