Deka N, Sharma M D, Mukerjee R
National Institute of Immunology, New Delhi, India.
J Virol. 1994 Dec;68(12):7810-5. doi: 10.1128/JVI.68.12.7810-7815.1994.
The agent(s) responsible for sporadic non-A, non-B hepatitis in humans was serially transmitted in rhesus monkeys by intravenous inoculation of the stool extract from a patient. A novel agent called HFV (hepatitis French [origin] virus) was present as 27- to 37-nm particles in the infectious stool extract. Hepatopathic lesions were noticed in infected monkeys during the acute phase of illness. The purified viral 27- to 37-nm particles consist of a double-stranded DNA of approximately 20 kb and are detected in infected monkey liver. Analysis of cell culture detects the approximately 20-kb-long viral DNA in stool samples from infected monkeys and sporadic enteric non-A, non-B hepatitis patients. Furthermore, the 27- to 37-nm viral particles were able to protect monkeys challenged with infectious stool extract. Our results indicate that 27- to 37-nm virus like particles are responsible for sporadic non-A, non-B hepatitis in rhesus monkeys.
通过静脉注射一名患者的粪便提取物，可将导致人类散发性非甲非乙型肝炎的病原体在恒河猴中连续传播。在具有传染性的粪便提取物中，一种名为HFV（法国[起源地]肝炎病毒）的新型病原体以27至37纳米的颗粒形式存在。在疾病急性期，受感染的猴子出现了肝病病变。纯化后的27至37纳米病毒颗粒由一条约20kb的双链DNA组成，并且在受感染的猴肝脏中被检测到。细胞培养分析在受感染猴子和散发性肠道非甲非乙型肝炎患者的粪便样本中检测到了约20kb长的病毒DNA。此外，27至37纳米的病毒颗粒能够保护受到传染性粪便提取物攻击的猴子。我们的结果表明，27至37纳米的病毒样颗粒是恒河猴散发性非甲非乙型肝炎的病因。
