多头绒泡菌的温度敏感突变体。I. 变形虫期的突变体

Temperature-sensitive mutants of the slime mould Physarum polycephalum. I. Mutants of the amoebal phase.

作者信息

Wheals A E, Grant W D, Jockusch B M

出版信息

Mol Gen Genet. 1976 Nov 24;149(1):111-4. doi: 10.1007/BF00275965.

Abstract

A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in clones. Some of these mutants appear to be cell-cycle stage specific.

摘要

已经设计出一种用于分离多头绒泡菌变形虫突变体的影印培养法。在亚硝基胍诱变后，温度敏感突变以每存活个体10⁻³的频率出现，这些突变是稳定的，但通常不在克隆中由这些变形虫形成的原质团中表达。其中一些突变体似乎具有细胞周期阶段特异性。