Cohen N D, Wallis D E, Neibergs H L, McElroy A P, McGruder E D, DeLoach J R, Corrier D E, Hargis B M
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Poult Sci. 1994 Aug;73(8):1276-81. doi: 10.3382/ps.0731276.
Drag-swab samples were collected from 18 poultry houses at 9 broiler farms. Fifty drag-swab samples were tested for Salmonella by microbiologic culture using selective enrichment and the polymerase chain reaction (PCR) with oligonucleotide primers specific for all members of the genus Salmonella. Drag-swab samples were tested for Salmonella using PCR before and after enrichment. Only one sample was positive by PCR prior to enrichment. Forty-seven of the drag-swabs samples tested after enrichment were positive for Salmonella using PCR, and 29 were positive by microbiologic culture. All but one of the culture-positive samples were positive by PCR; this discordant sample was classified as indeterminate by PCR. Salmonella was identified in houses from all nine farms by PCR and eight of nine farms by microbiologic culture. Salmonella was found in all 18 houses by PCR and in 15 of 18 houses by microbiologic culture. In this study, PCR was significantly (P < .001) more sensitive than culture for environmental monitoring of Salmonella using drag-swabs.
从9个肉鸡场的18个禽舍采集拖拭样本。使用选择性增菌和针对沙门氏菌属所有成员的寡核苷酸引物进行聚合酶链反应（PCR），对50个拖拭样本进行沙门氏菌微生物培养检测。在增菌前后使用PCR对拖拭样本进行沙门氏菌检测。增菌前只有1个样本通过PCR呈阳性。增菌后检测的47个拖拭样本通过PCR检测出沙门氏菌呈阳性，29个通过微生物培养呈阳性。除1个样本外，所有培养阳性样本通过PCR均呈阳性；这个不一致的样本通过PCR被分类为不确定。通过PCR在所有9个农场的禽舍中均检测到沙门氏菌，通过微生物培养在9个农场中的8个检测到。通过PCR在所有18个禽舍中均发现沙门氏菌，通过微生物培养在18个禽舍中的15个发现。在本研究中，使用拖拭样本对沙门氏菌进行环境监测时，PCR的敏感性显著高于培养（P < .001）。
