Shatkin A A, Beskina S R, Pankrstova V N, Popov V L, Zakharova N A
Zh Mikrobiol Epidemiol Immunobiol. 1976 Sep(9):74-8.
The authors modified and approbated the direct immunoperoxidase method for the indication in the infected L cells of the halprowia (chlamydia) antigens--etiological agents of trachoma, ocular and urogenital forms of paratrachoma, arthritis, meningopneumonia and enzootic abortion of sheep. Different types of localization of the group-specific halprowia antigen were revealed in the cytoplasm of the affected cells by light microscopy; electron microscopy demonstrated localization of this antigen in the membrane of the cell wall of the elementary and initial bodies. Specificity of the test-system used, no lesser sensitivity than the sensitivity of direct immunofluorescent method of indication of the same microbies, with a possibility of detection of stably stained antigens by widely accessible light microscopy underly practical usefulness of direct immunoperoxidase method of the diagnosis of halprowiosis (chlamydiosis) of man and animals.
作者改良并认可了直接免疫过氧化物酶法,用于在感染的L细胞中检测嗜衣原体(衣原体)抗原——沙眼、眼及泌尿生殖系统型副沙眼、关节炎、脑膜肺炎和绵羊地方性流产的病原体。通过光学显微镜在受感染细胞的细胞质中发现了群特异性嗜衣原体抗原的不同定位类型;电子显微镜显示该抗原定位于原体和始体细胞壁的膜中。所使用的检测系统具有特异性,其灵敏度不低于检测相同微生物的直接免疫荧光法,且有可能通过广泛使用的光学显微镜检测到稳定染色的抗原,这些构成了直接免疫过氧化物酶法在诊断人和动物嗜衣原体病(衣原体病)方面实际应用的基础。