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[血培养:检测慢性恰加斯病阶段恰加斯病患者中克氏锥虫的敏感技术]

[Hemoculture: sensitive technique in the detection of Trypanosoma cruzi in chagasic patients in the chronic phase of Chagas disease].

作者信息

Luz Z M, Coutinho M G, Cançado J R, Krettli A U

机构信息

Centro de Pesquisas René Rachou/Fundação Oswaldo Cruz e Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, MG.

出版信息

Rev Soc Bras Med Trop. 1994 Jul-Sep;27(3):143-8. doi: 10.1590/s0037-86821994000300004.

Abstract

The sensitivity of hemocultures, performed once or three times, was investigated in 52 patients in the chronic phase of Chagas disease. Modifications were introduced in the technique such as, processing the blood more rapidly, gently homogenizing the cultures and examining them after 120 days of culture. Our results show a high percent age of positivity i.e. 79% and 94% of patients submitted, respectively, to one or three tests. No significant differences related to the patients age were detected, which varied from 14 to 82 years old. Our results demonstrate that hemoculture is a sensitive method for the parasitological diagnosis of Chagas disease and is ideal for monitoring cure in treated patients.

摘要

我们对52例恰加斯病慢性期患者进行了血培养敏感性研究,血培养分别进行一次或三次。在技术上做了一些改进,比如更快地处理血液、轻柔地使培养物均匀化,并在培养120天后进行检查。我们的结果显示阳性率很高,即分别接受一次或三次检测的患者中,阳性率为79%和94%。未检测到与患者年龄(14至82岁)相关的显著差异。我们的结果表明,血培养是恰加斯病寄生虫学诊断的一种敏感方法,也是监测治疗患者治愈情况的理想方法。

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