用酶联免疫吸附测定法（ELISA）检测母马子宫冲洗液中的马α-1蛋白酶抑制剂。

Measurement by ELISA of equine alpha-1-proteinase inhibitor in uterine flushings from mares.

作者信息

Scudamore C L, Pemberton A D, Miller H R, McDonnell A M, Thomson S R, Dawson A, Watson E D

机构信息

Department of Veterinary Clinical Studies, Veterinary Field Station, Roslin, Midlothian.

出版信息

Res Vet Sci. 1994 Jul;57(1):45-52. doi: 10.1016/0034-5288(94)90080-9.

DOI:10.1016/0034-5288(94)90080-9

PMID:7973092

Abstract

An enzyme linked immunosorbent assay (ELISA) was developed and used to estimate the concentrations of the serine proteinase inhibitor, alpha-1 proteinase inhibitor (API), in uterine flushings recovered from mares at different stages of the oestrous cycle and before and after the induction of experimental endometritis. There was a significant increase in the concentrations of API and albumin relative to total protein in flushings recovered during oestrus compared with dioestrus but no difference was observed in the concentrations of these proteins relative to total protein before and after the induction of endometritis. A regression analysis revealed a significant correlation between the concentrations of albumin and API in the flushings examined, suggesting that the API was derived entirely from serum and was not produced locally in the uterus.

摘要

开发了一种酶联免疫吸附测定法（ELISA），用于估计从处于发情周期不同阶段以及实验性子宫内膜炎诱导前后的母马子宫冲洗液中丝氨酸蛋白酶抑制剂α-1蛋白酶抑制剂（API）的浓度。与发情间期相比，发情期回收的冲洗液中API和白蛋白相对于总蛋白的浓度显著增加，但在子宫内膜炎诱导前后，这些蛋白质相对于总蛋白的浓度没有差异。回归分析显示，所检测的冲洗液中白蛋白和API的浓度之间存在显著相关性，表明API完全来源于血清，并非子宫局部产生。