Hu L K, Huh K, Gragoudas E S, Young L H
Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston 02114.
Retina. 1994;14(3):264-9. doi: 10.1097/00006982-199414030-00014.
To establish an animal model of pigmented choroidal melanoma.
Four melanoma cell lines originally isolated from melanotic tumors (B16F10, RPMI 1846, OCM 1, and IIB) were used to establish choroidal melanomas in 105 rabbits; 88 animals were immunosuppressed with cyclosporine. Tumor cells were implanted transclerally and examined with indirect ophthalmoscopy, ultrasound, and photography.
Characteristic growth patterns were noted for each cell line. Animal cell lines typically produced choroidal tumors 3 to 4 mm in height within 2 weeks; human cell lines took an additional 7 to 10 days to achieve tumors of similar height. Tumors of heaviest pigmentation were generated consistently with the B16F10 cells, and with the other three cell lines only mild pigmentation was observed. Tumor shape varied depending on the source of implantation: diffuse, flat tumors were observed when cell suspensions were implanted, and nodular tumors were obtained with tumor fragments. Histopathologically, lesions were highly cellular, with rich vascularity and large numbers of mitotic figures.
As the majority of human uveal melanomas are pigmented, the added feature of pigmentation associated with this model makes it more suitable for evaluating the role of newly developed phototherapies in the management of uveal melanoma.
建立色素性脉络膜黑色素瘤动物模型。
使用最初从黑色素瘤中分离出的四种黑色素瘤细胞系（B16F10、RPMI 1846、OCM 1和IIB）在105只兔子中建立脉络膜黑色素瘤；88只动物用环孢素进行免疫抑制。通过巩膜植入肿瘤细胞，并采用间接检眼镜、超声和摄影进行检查。
每种细胞系都有其特征性的生长模式。动物细胞系通常在2周内产生高度为3至4毫米的脉络膜肿瘤；人类细胞系则需要额外7至10天才能达到相似高度的肿瘤。B16F10细胞始终产生色素沉着最重的肿瘤，而其他三种细胞系仅观察到轻度色素沉着。肿瘤形状因植入来源而异：植入细胞悬液时观察到弥漫性扁平肿瘤，植入肿瘤碎片时获得结节性肿瘤。组织病理学上，病变细胞高度密集，血管丰富，有大量有丝分裂象。
由于大多数人类葡萄膜黑色素瘤是色素性的，该模型中色素沉着这一附加特征使其更适合评估新开发的光疗法在葡萄膜黑色素瘤治疗中的作用。
