Liggett P E, Lo G, Pince K J, Rao N A, Pascal S G, Kan-Mitchel J
Department of Ophthalmology, University of Southern California School of Medicine, Los Angeles.
Graefes Arch Clin Exp Ophthalmol. 1993;231(1):15-20. doi: 10.1007/BF01681695.
Cell lines established from the biopsy specimen of a patient with a spindle B choroidal melanoma were transplanted into the posterior choroid of 30 immunosuppressed, pigmented New Zealand rabbits. Growth of the tumor xenografts could be seen 7 to 10 days after transplantation. Tumor xenografts were reproducible and reached an average size of 5 mm in height and 8 mm in their basal dimension by 4 weeks. Histopathology of the original tumor revealed primarily spindle B cells, while the tumor xenografts contained epithelioid, spindle B, and clear cell melanoma cells. Of particular interest was the presence of an extensive and intact vasculature and the absence of a capsule surrounding the lesions in situ. The use of human uveal melanoma cells, the ease of transplantation, and the posterior location of the tumor may make this animal model of use in studies on new diagnostic and therapeutic modalities.
从一名患有梭形B脉络膜黑色素瘤患者的活检标本中建立的细胞系，被移植到30只免疫抑制的有色新西兰兔的脉络膜后部。移植后7至10天可见肿瘤异种移植物生长。肿瘤异种移植物可重复出现，到4周时平均高度达5毫米，基底直径达8毫米。原发肿瘤的组织病理学显示主要为梭形B细胞，而肿瘤异种移植物含有上皮样、梭形B和透明细胞黑色素瘤细胞。特别令人感兴趣的是存在广泛且完整的脉管系统，以及原位病变周围无包膜。人葡萄膜黑色素瘤细胞的使用、移植的便利性以及肿瘤的后部位置，可能使这种动物模型可用于新诊断和治疗方式的研究。
