Gad S C
Gad Consulting Services, Raleigh, NC 27608.
Toxicology. 1994 Sep 22;93(1):33-46. doi: 10.1016/0300-483x(94)90194-5.
The mouse ear swelling test (MEST) was developed in the early 1980s to provide a lower cost, shorter and objectively graded alternative to the existing guinea pig based tests for delayed contact hypersensitivity. In the ensuing time, the test design has been modified and its use has been extended to additional applications (phototoxicity and photosensitisation screening and as a mechanistic tool in studying tumor promoting agents) and to evaluating a broader range of test materials (treated fabrics, medical devices, environmental pollutants, specialty chemicals, drugs, etc.) in the hands of new investigators. Likewise, other murine based tests have been developed. The MEST and one other murine test (the local lymph node assay, or LLNA) have also now been included in the lists of regulatorily accepted sensitization test systems. The current version of the MEST protocol is presented here, along with the rationale for changes, a review of evaluations and interlaboratory trials and an overview of the range of uses to which it has been applied.
小鼠耳肿胀试验（MEST）于20世纪80年代初开发，旨在为现有的基于豚鼠的迟发型接触性超敏反应试验提供一种成本更低、时间更短且客观分级的替代方法。在随后的时间里，该试验设计得到了改进，其应用范围也扩展到了其他领域（光毒性和光敏性筛选以及作为研究肿瘤促进剂的机制工具），并且在新的研究人员手中用于评估更广泛的试验材料（经处理的织物、医疗器械、环境污染物、特种化学品、药物等）。同样，也开发了其他基于小鼠的试验。MEST和另一种基于小鼠的试验（局部淋巴结试验，或LLNA）现在也已被列入监管机构认可的致敏试验系统列表中。本文介绍了MEST方案的当前版本，以及更改的理由、评估和实验室间试验的综述，以及其应用范围的概述。
