Bäumer W, Bizikova P, Jacob M, Linder K E
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, NC State University, Raleigh, NC, USA.
Department of Clinical Sciences, College of Veterinary Medicine, NC State University, Raleigh, NC, USA.
J Appl Microbiol. 2017 Feb;122(2):331-337. doi: 10.1111/jam.13362. Epub 2017 Jan 4.
Pyoderma, predominantly associated with Staphylococcus pseudintermedius, is a common skin infection of dogs that typically requires long-lasting treatments, complicated by increasing antimicrobial resistance. To investigate new treatment strategies, we aimed at establishing a dog model of pyoderma that closely mimics the natural disease.
We inoculated six laboratory beagles with a methicillin-susceptible strain of S. pseudintermedius. One millilitre of approximately 10 , 10 , 10 CFU per ml was topically applied onto clipped and tape stripped area of dog skin, which was then treated with a dermaroller (microneedle size: 500 μm) immediately after administration. Dogs were monitored daily, suspect pustules were cultured for S. pseudintermedius and evaluated by cytological and histopathological methods. After 24 h, all dogs developed papules and pustules at all three bacterial inoculation sites, which worsened over the next 48 h. Cytological samples of all skin lesions revealed neutrophils with intracellular cocci. Histopathology confirmed subcorneal neutrophilic pustular dermatitis with intralesional cocci and acantholytic keratinocytes, consistent with superficial pyoderma. Staphylococcus pseudintermedius was isolated from pustules of all dogs and confirmed to be the inoculating strain. The results were replicated in all dogs after a wash out period of 6 weeks.
These data demonstrate the feasibility of establishing a dog model of pyoderma.
The new model can be used to evaluate novel prevention and treatment options for canine pyoderma.
脓疱性皮炎主要与中间型假葡萄球菌有关，是犬类常见的皮肤感染，通常需要长期治疗，且因抗菌药物耐药性增加而变得复杂。为了研究新的治疗策略，我们旨在建立一种能紧密模拟自然疾病的犬脓疱性皮炎模型。
我们给6只实验用比格犬接种了对甲氧西林敏感的中间型假葡萄球菌菌株。将每毫升约10⁶、10⁷、10⁸CFU的1毫升菌液局部涂抹在犬只已剃毛并经胶带剥离处理的皮肤区域，给药后立即用微针滚轮（微针尺寸：500μm）进行处理。每天对犬只进行监测，对疑似脓疱进行中间型假葡萄球菌培养，并通过细胞学和组织病理学方法进行评估。24小时后，所有犬只在三个细菌接种部位均出现丘疹和脓疱，并在接下来的48小时内恶化。所有皮肤病变的细胞学样本均显示有含细胞内球菌的中性粒细胞。组织病理学证实为角层下中性粒细胞脓疱性皮炎，伴有病灶内球菌和棘层松解性角质形成细胞，与浅表脓疱性皮炎一致。从所有犬只的脓疱中分离出中间型假葡萄球菌，并证实为接种菌株。在6周的洗脱期后，所有犬只均重复出现了该结果。
这些数据证明了建立犬脓疱性皮炎模型的可行性。
新模型可用于评估犬脓疱性皮炎的新型预防和治疗方案。
