Hotomi M, Samukawa T, Yamanaka N
Department of Otorhinolaryngology, Wakayama Medical College, Japan.
Acta Otolaryngol. 1994 Jul;114(4):406-9. doi: 10.3109/00016489409126078.
Interleukin-8 induces chemotaxis of neutrophils, basophils and T-lymphocytes, releases intracellular enzymes from neutrophils and histamine from basophils, and regulates the adhesion of neutrophils. In this study, using an enzyme-linked immunosorbent assay, we evaluated 33 middle ear effusions (MEEs) for levels of IL-8. The mean level of IL-8 in MEEs from children with OME was 616.7 +/- 211.0 pg/mgTP, while that of adults was 197.4 +/- 66.7 pg/mgTP. With respect to MEE types, the mean level of IL-8 in serous effusion was lower than that in two other types of MEEs (mucoserous and serous). These results suggest that inflammatory reaction in the middle ear cavity of children with OME is different from that of adults and that the pathogenesis of OME in children may differ from that in adults. Determination of IL-8 concentration in MEEs may help to illuminate the pathogenesis of OME.
白细胞介素-8可诱导中性粒细胞、嗜碱性粒细胞和T淋巴细胞的趋化作用，促使中性粒细胞释放细胞内酶以及嗜碱性粒细胞释放组胺，并调节中性粒细胞的黏附。在本研究中，我们采用酶联免疫吸附测定法评估了33份中耳积液（MEE）中的白细胞介素-8水平。患分泌性中耳炎（OME）儿童的中耳积液中白细胞介素-8的平均水平为616.7±211.0 pg/mgTP，而成人的该水平为197.4±66.7 pg/mgTP。就中耳积液类型而言，浆液性积液中白细胞介素-8的平均水平低于其他两种类型的中耳积液（黏液浆液性和浆液性）。这些结果表明，患OME儿童中耳腔的炎症反应与成人不同，儿童OME的发病机制可能也与成人不同。测定中耳积液中白细胞介素-8的浓度可能有助于阐明OME的发病机制。
