Nassif P S, Simpson S Q, Izzo A A, Nicklaus P J
Division of Otolaryngology-Head and Neck Surgery, University of New Mexico Health Sciences Center, Albuquerque 87131-5341, U.S.A.
Laryngoscope. 1997 Sep;107(9):1223-7. doi: 10.1097/00005537-199709000-00012.
Interleukin-8 (IL-8), a potent neutrophilic chemoattractant and inflammatory cytokine, is present in middle ear effusions (MEEs) of children with otitis media and is thought to be responsible for the accumulation of neutrophils in MEEs. We hypothesized that IL-8 concentration predicts the total number and proportion of neutrophils in MEEs. IL-8 concentration and total and differential cell counts were measured in MEEs of children undergoing tympanostomy tube placement for otitis media. IL-8 was present in 80 (98%) of 82 effusions. The mean +/- SEM value for IL-8 was 7342 +/- 847 pg/mL. The mean +/- SEM count and percentage of neutrophils were 1.34 x 10(6) +/- 3.44 x 10(5) and 70.6 +/- 3.1%, respectively. IL-8 concentrations correlated positively with the total number (r = +0.30; P = 0.02) and percentage of neutrophils (r = +0.32; P = 0.01) in the effusion. Additionally, purulent effusions had greater IL-8 concentrations (P = 0.003) and greater neutrophil count (P = 0.03) than mucoid or serous effusions. We conclude that IL-8 is consistently present in MEEs of children and IL-8 concentration predicts the total number and proportion of neutrophils. Furthermore, IL-8 concentration and the total number of neutrophils correlate positively with the type of effusion. These results support the hypothesis that IL-8 recruits neutrophils to the middle ear in MEEs.
白细胞介素-8（IL-8）是一种强效的嗜中性粒细胞趋化因子和炎性细胞因子，存在于患有中耳炎的儿童的中耳积液（MEE）中，被认为是导致MEE中嗜中性粒细胞积聚的原因。我们假设IL-8浓度可预测MEE中嗜中性粒细胞的总数和比例。对因中耳炎接受鼓膜置管术的儿童的MEE进行了IL-8浓度以及细胞总数和分类计数的测量。82例积液中有80例（98%）存在IL-8。IL-8的平均±标准误值为7342±847 pg/mL。嗜中性粒细胞的平均±标准误计数和百分比分别为1.34×10⁶±3.44×10⁵和70.6±3.1%。IL-8浓度与积液中嗜中性粒细胞的总数（r = +0.30；P = 0.02）和百分比（r = +0.32；P = 0.01）呈正相关。此外，脓性积液的IL-8浓度（P = 0.003）和嗜中性粒细胞计数（P = 0.03）均高于黏液性或浆液性积液。我们得出结论，IL-8始终存在于儿童的MEE中，且IL-8浓度可预测嗜中性粒细胞的总数和比例。此外，IL-8浓度和嗜中性粒细胞总数与积液类型呈正相关。这些结果支持了IL-8在MEE中招募嗜中性粒细胞至中耳的假设。
