Matsumura A, Shibata Y, Nakagawa K, Nose T
Department of Neurosurgery, University of Tsukuba, Ibaraki, Japan.
Acta Neurochir Suppl (Wien). 1994;60:356-8. doi: 10.1007/978-3-7091-9334-1_96.
To achieve a tissue-specific enhancement in diagnosis of brain tumor, a magnetic resonance imaging (MRI) study was performed using conjugate of Gd-DTPA and monoclonal antibody (MoAb) against 9L glioma cells. Fisher 344 strain rats were used for this study. MoAb against 9L glioma cells was conjugated with Gd-DTPA according to the method of Hnatowich et al. (1983) and used for the MRI study. The gadolinium (Gd) concentration in the Gd-MoAb injected to the rats was 0.01-0.03 mmol/kg. The enhancement effect increased gradually and persisted for 24 hours after the injection. This was longer than Gd-DTPA, which showed a peak of enhancement effect within 30 minutes after injection and was washed out within 120 min. This result was compatible with scintigraphy studies using 125I labeled anti 9L monoclonal antibody, in which the accumulation of the 125I antibody increased at 24, 48 and 72 hours after the injection. By using tumor-specific contrast agents such as Gd-MoAb, it may be possible to differentiate among tumor, perifocal edema and radiation injury.
为了在脑肿瘤诊断中实现组织特异性增强，使用钆-二乙三胺五乙酸（Gd-DTPA）与抗9L胶质瘤细胞单克隆抗体（MoAb）的偶联物进行了磁共振成像（MRI）研究。本研究使用了Fisher 344品系大鼠。根据Hnatowich等人（1983年）的方法，将抗9L胶质瘤细胞的MoAb与Gd-DTPA偶联，并用于MRI研究。注入大鼠体内的钆（Gd）-MoAb中的钆浓度为0.01 - 0.03 mmol/kg。增强效果逐渐增加，并在注射后持续24小时。这比Gd-DTPA的持续时间长，Gd-DTPA在注射后30分钟内显示增强效果峰值，并在120分钟内被清除。该结果与使用125I标记的抗9L单克隆抗体的闪烁显像研究结果相符，在该研究中，125I抗体的积累在注射后24、48和72小时增加。通过使用肿瘤特异性造影剂，如Gd-MoAb，有可能区分肿瘤、灶周水肿和放射性损伤。
