Pecivová J, Drábiková K, Nosál R
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Agents Actions. 1994 Jun;41 Spec No:C43-4. doi: 10.1007/BF02007759.
The effect of chloroquine (CQ) on phospholipid turnover and de novo synthesis in isolated rat mast cells (IRMC) was studied by determining the incorporation of 32P and 14C-glycerol into IRMC phospholipids. Incubation of mast cells with chloroquine increased 32P incorporation into PI and PS whilst it decreased 32P incorporation into PC, PE and PA. In mast cells pretreated with CQ and subsequently stimulated with compound 48/80, 32P incorporation into PI, PS and PA fractions was enhanced, while it was decreased into PC and PE, in comparison to 48/80 stimulated IRMC. 14C-glycerol incorporation into total IRMC phospholipids was not significantly changed by CQ and compound 48/80 treatment and neither was any dose-dependent effect of CQ on individual phospholipids detected. Our results indicate that chloroquine, similarly to other cationic amphiphilic drugs, may alter membrane PL turnover without changing de novo synthesis of phospholipids.
通过测定32P和14C-甘油掺入分离的大鼠肥大细胞（IRMC）磷脂中的情况，研究了氯喹（CQ）对IRMC中磷脂周转和从头合成的影响。用氯喹孵育肥大细胞可增加32P掺入PI和PS，同时减少32P掺入PC、PE和PA。与用化合物48/80刺激的IRMC相比，在用CQ预处理并随后用化合物48/80刺激的肥大细胞中，32P掺入PI、PS和PA组分增强，而掺入PC和PE减少。CQ和化合物48/80处理对14C-甘油掺入IRMC总磷脂的影响不显著，也未检测到CQ对单个磷脂的任何剂量依赖性效应。我们的结果表明，与其他阳离子两亲性药物类似，氯喹可能改变膜磷脂周转，而不改变磷脂的从头合成。
