Kelley P K, McClain K L
Department of Pediatrics, Baylor College of Medicine, Houston, TX.
Am J Hematol. 1994 Dec;47(4):325-7. doi: 10.1002/ajh.2830470415.
Human herpesvirus type 6 DNA derived from human breath was discovered to contaminate PCR reactions during routine reaction preparation. Parallel PCR experiments were conducted in which expiratory secretions were blocked by a surgical mask, while others were performed without any attempt to circumvent respiratory contamination. The experimenter was previously determined to harbor HHV-6 DNA in the saliva. All reactions in which expiration was obstructed were negative for HHV-6 DNA via PCR. Reactions in which there was no attempt to obstruct respiratory secretions were positive for HHV-6 DNA. These data suggest that PCR assays investigating the presence of HHV-6 may be highly susceptible to contamination from the experimenter leading to false positive results.
在常规反应准备过程中，发现源自人类呼出气体的人疱疹病毒6型DNA会污染聚合酶链反应（PCR）。进行了平行PCR实验，其中一组实验中用外科口罩阻挡呼气分泌物，而另一组实验未采取任何措施避免呼吸道污染。实验者之前被确定唾液中携带人疱疹病毒6型DNA。所有呼气被阻挡的反应经PCR检测人疱疹病毒6型DNA均为阴性。未采取措施阻挡呼吸道分泌物的反应中，人疱疹病毒6型DNA为阳性。这些数据表明，检测人疱疹病毒6型存在的PCR检测可能极易受到实验者的污染，从而导致假阳性结果。
