Clark D A, Kidd I M, Collingham K E, Tarlow M, Ayeni T, Riordan A, Griffiths P D, Emery V C, Pillay D
Department of Virology, Royal Free Hospital School of Medicine, London.
Arch Dis Child. 1997 Jul;77(1):42-5. doi: 10.1136/adc.77.1.42.
Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in febrile children by qualitative and quantitative polymerase chain reaction (PCR) assays. Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile infants, but none of seven non-febrile controls, were identified with primary infections (three HHV-6, three HHV-7). These children had significantly higher viral loads in PBMC (HHV-6, median 24213 genomes/10(6) PBMC; HHV-7, median 6,040,000 genomes/10(6) PBMC) than DNA-aemic, saliva PCR positive children (HHV-6, median 1606 genomes/10(6) PBMC, p < 0.01; HHV-7, median 7089 genomes/ 10(6) PBMC, p < 0.05). Viral DNA was detected in serum by PCR in only 50% of primary infections. All three children with primary HHV-7 infection had febrile convulsions. Thus PCR, including quantitative assays, may identify primary HHV-6 and HHV-7 infections when an appropriate combination of clinical specimens is used.
通过定性和定量聚合酶链反应（PCR）检测，在发热儿童中发现了原发性人类疱疹病毒6型（HHV-6）和7型（HHV-7）感染。诊断基于外周血单个核细胞（PBMC）而非唾液中病毒DNA的差异检测。41名发热婴儿中有6名被确诊为原发性感染（3例HHV-6，3例HHV-7），而7名非发热对照中无一例感染。这些儿童PBMC中的病毒载量（HHV-6，中位数为24213个基因组/10⁶PBMC；HHV-7，中位数为6040000个基因组/10⁶PBMC）显著高于DNA血症、唾液PCR阳性的儿童（HHV-6，中位数为1606个基因组/10⁶PBMC，p<0.01；HHV-7，中位数为7089个基因组/[10⁶PBMC，p<0.05]）。仅50%的原发性感染通过PCR在血清中检测到病毒DNA。所有3例原发性HHV-7感染儿童均发生了热性惊厥。因此，当使用适当组合的临床标本时，包括定量检测在内的PCR可能有助于识别原发性HHV-6和HHV-7感染。
