Vo-Dinh T, Houck K, Stokes D L
Health Sciences Research Division, Oak Ridge National Laboratory, Tennessee 37831-6101.
Anal Chem. 1994 Oct 15;66(20):3379-83. doi: 10.1021/ac00092a014.
We report for the first time a new type of DNA gene probe based on surface-enhanced Raman scattering (SERS) label detection. The surface-enhanced Raman gene (SERG) probes do not require the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The SERG probe can be used to detect DNA biotargets (e.g., gene sequences, bacteria, viral DNA fragments) via hybridization to DNA sequences complementary to that probe. The analytical figures of merit and its applications in environmental and biomedical areas are discussed.
我们首次报道了一种基于表面增强拉曼散射（SERS）标记检测的新型DNA基因探针。表面增强拉曼基因（SERG）探针无需使用放射性标记，在提供灵敏度和选择性方面具有巨大潜力。SERG探针可通过与该探针互补的DNA序列杂交，用于检测DNA生物靶点（如基因序列、细菌、病毒DNA片段）。本文讨论了其分析性能指标及其在环境和生物医学领域的应用。
