Semenova L E, Sherstobitova T S, Gorokhova I B
Antibiot Khimioter. 1994 Feb-Mar;39(2-3):3-8.
It was shown possible to improve the process of lincomycin production by using batch-type feeding of the carbohydrate substrates during the biosynthesis. The optimal composition of the fermentation medium with lower concentrations of the carbohydrates was developed and batch-type feeding of the substrates was applied. The conditions of the substrate feeding were developed under laboratory conditions. Solutions of glucose or sugar supplemented with potassium sulfate were used for the feeding. The antibiotic yield under the optimal conditions of the substrate feeding and the process control was higher than that under the conditions of the batch fermentation by 23-24 per cent.
结果表明，在林可霉素生物合成过程中采用碳水化合物底物分批式补料可以改进其生产工艺。开发了碳水化合物浓度较低的发酵培养基的最佳组成，并应用了底物分批式补料。底物补料的条件是在实验室条件下确定的。补料使用补充了硫酸钾的葡萄糖或糖溶液。在底物补料和过程控制的最佳条件下，抗生素产量比分批发酵条件下高23% - 24%。
