Hellmann E, Stanzel C, Tonkaboni T F
Institut für Mikrobiologie und Tierseuchen, Freien Universität Berlin.
Berl Munch Tierarztl Wochenschr. 1994 Sep;107(9):308-13.
Polyclonal rabbit antisera against 4 B. cereus strains were selected from a total of 9 B. cereus antisera and pooled. This serum agglutinated all available B. cereus strains (n = 63) at a titre > or = 1:64 when tested by a slide co-agglutination reaction. One hundred and thirty-six Bacillus strains belonging to 17 other species reacted with higher serum concentrations only (titres mainly < or = 1:16). A pooled antiserum comprised of two B. licheniformis antisera and two B. subtilis antisera agglutinated all 43 B. licheniformis strains and all 38 B. subtilis strains at a titre > or = 1:128, but one strain each of the species B. pumilus, B. macerans and B. fastidiosus was also agglutinated at a titre 1:128.
从总共9种蜡样芽孢杆菌抗血清中挑选出针对4种蜡样芽孢杆菌菌株的多克隆兔抗血清并进行混合。当通过玻片协同凝集反应检测时,这种血清能以≥1:64的滴度凝集所有可用的蜡样芽孢杆菌菌株(n = 63)。属于其他17个物种的136株芽孢杆菌菌株仅在血清浓度较高时发生反应(滴度主要≤1:16)。由两种地衣芽孢杆菌抗血清和两种枯草芽孢杆菌抗血清组成的混合抗血清能以≥1:128的滴度凝集所有43株地衣芽孢杆菌菌株和所有38株枯草芽孢杆菌菌株,但短小芽孢杆菌、浸麻芽孢杆菌和苛求芽孢杆菌各有1株也能以1:128的滴度被凝集。