O'Brien J R, George N M
Department of Pathology, Royal Brisbane Hospital, Herston, Queensland, Australia.
Microbios. 1997;90(363):71-8.
Discs containing the pteridine compound O129 at various concentrations may be useful in differentiating the following closely related species belonging to the genus Bacillus, viz. B. subtilis, B. licheniformis and B. pumilus; B. polymyxa and B. macerans; together with B. cereus and its subspecies B. cereus var. mycoides. At concentrations of 10 micrograms O129, the ATCC type strain of B. subtilis was resistant whereas B. licheniformis and B. pumilus were sensitive. However, B. subtilis was sensitive to O129 at 150 micrograms. Similar reactions differentiated the ATCC type strains of B. polymyxa and B. macerans. The ATCC type strain of B. cereus was resistant to O129 at both 150 micrograms and 10 micrograms, but its subspecies B. cereus var. mycoides (ATCC 28) was partially sensitive at 150 micrograms concentration. When clinical isolates of B. cereus var. mycoides were subsequently tested, this partial sensitivity was found to be a variable characteristic.
含有不同浓度蝶啶化合物O129的圆片可能有助于区分以下属于芽孢杆菌属的密切相关物种，即枯草芽孢杆菌、地衣芽孢杆菌和短小芽孢杆菌；多粘芽孢杆菌和浸麻芽孢杆菌；以及蜡样芽孢杆菌及其亚种蜡样芽孢杆菌蕈状变种。在O129浓度为10微克时，枯草芽孢杆菌的ATCC模式菌株具有抗性，而地衣芽孢杆菌和短小芽孢杆菌敏感。然而，枯草芽孢杆菌在150微克O129时敏感。类似的反应区分了多粘芽孢杆菌和浸麻芽孢杆菌的ATCC模式菌株。蜡样芽孢杆菌的ATCC模式菌株在150微克和10微克O129时均具有抗性，但其亚种蜡样芽孢杆菌蕈状变种（ATCC 28）在150微克浓度时部分敏感。随后对蜡样芽孢杆菌蕈状变种的临床分离株进行测试时，发现这种部分敏感性是一种可变特征。
