类风湿关节炎、干燥综合征和系统性红斑狼疮中非T细胞在体内产生白细胞介素-10。B淋巴细胞功能亢进和自身免疫的一种潜在机制。
In vivo production of interleukin-10 by non-T cells in rheumatoid arthritis, Sjögren's syndrome, and systemic lupus erythematosus. A potential mechanism of B lymphocyte hyperactivity and autoimmunity.
作者信息
Llorente L, Richaud-Patin Y, Fior R, Alcocer-Varela J, Wijdenes J, Fourrier B M, Galanaud P, Emilie D
机构信息
Department of Immunology and Rheumatology, Instituto Nacional de la Nutrición Salvador Zubirán, México, D.F., México.
出版信息
Arthritis Rheum. 1994 Nov;37(11):1647-55. doi: 10.1002/art.1780371114.
OBJECTIVE
Interleukin-10 (IL-10) is a potent stimulator of B lymphocytes in vitro. In vivo dysregulation of IL-10 gene expression was therefore analyzed in patients with rheumatoid arthritis (RA), primary Sjögren's syndrome (SS), and systemic lupus erythematosus (SLE).
METHODS
Spontaneous production of IL-10 by peripheral blood mononuclear cells was measured using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay in untreated patients with either RA (n = 10), SS (n = 10), or SLE (n = 10), and in 15 normal control subjects.
RESULTS
IL-10 production was dramatically higher in RA, SS, and SLE patients than in controls. In each group, both B lymphocytes and monocytes, but not T lymphocytes, produced IL-10.
CONCLUSION
IL-10 production is increased in RA, SS, and SLE. It may play a role in B lymphocyte hyperactivity and in the development of autoimmunity.
目的
白细胞介素-10（IL-10）在体外是B淋巴细胞的强效刺激因子。因此，对类风湿关节炎（RA）、原发性干燥综合征（SS）和系统性红斑狼疮（SLE）患者体内IL-10基因表达的失调情况进行了分析。
方法
采用逆转录聚合酶链反应和酶联免疫吸附测定法，检测了未经治疗的RA患者（n = 10）、SS患者（n = 10）、SLE患者（n = 10）以及15名正常对照者外周血单个核细胞自发产生IL-10的情况。
结果
RA、SS和SLE患者体内IL-10的产生显著高于对照组。在每组中，B淋巴细胞和单核细胞均可产生IL-10，而T淋巴细胞则不能。
结论
RA、SS和SLE患者体内IL-10的产生增加。它可能在B淋巴细胞的过度活跃以及自身免疫的发展过程中发挥作用。
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