大肠杆菌O20:K84:H26产生的荚膜多糖的结构解析
Structural elucidation of the capsular polysaccharide produced by Escherichia coli O20 : K84 : H26.
作者信息
Whittaker D V, Parolis L A, Parolis H
机构信息
School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
出版信息
Carbohydr Res. 1994 Sep 15;262(2):323-34. doi: 10.1016/0008-6215(94)84188-8.
The primary structure of the acidic capsular antigen produced by E. coli K84 was shown by glycose and methylation analysis, and by 1D and 2D 1H and 13C NMR studies of the polysaccharide and an oligosaccharide produced by lithium-ethylenediamine degradation of the polysaccharide, to be comprised of linear hexasaccharide repeating units of the following structure: -->4)alpha-D-GaI pA-(1-->3)-alpha-D-Man p-(1-->4)-beta-D-GIc p-(1-->3)-alpha- D-Glc pNAc-(1-->2)-beta-D-Man p-(1-->3)-beta-D-Man pNAc-(1-->.
通过葡萄糖和甲基化分析，以及对多糖和由多糖经锂 - 乙二胺降解产生的寡糖进行的一维和二维氢谱及碳谱核磁共振研究表明，大肠杆菌K84产生的酸性荚膜抗原的一级结构由以下结构的线性六糖重复单元组成：-->4)α-D-半乳糖醛酸-(1→3)-α-D-甘露糖-(1→4)-β-D-葡萄糖-(1→3)-α-D-葡萄糖胺-(1→2)-β-D-甘露糖-(1→3)-β-D-甘露糖胺-(1→ 。
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