Goswami S, Sarkar S, Basu J, Kundu M, Chakrabarti P
Department of Chemistry, Bose Institute, Calcutta, India.
FEBS Lett. 1994 Nov 28;355(2):183-6. doi: 10.1016/0014-5793(94)01203-2.
Pathogenic Mycobacteria colonize host macrophages. Attachment of these organisms to macrophages is the preliminary step prior to invasion of the macrophages by the bacteria. Western blot confirmed that walls of Mycobacterium avium and Mycobacterium tuberculosis contain molecules which are immunologically related to mycotin, a lectin found in Mycobacterium smegmatis. We have demonstrated that the adherence of Mycobacteria to macrophages is significantly inhibited by anti-mycotin antibody or the mycotin-specific sugar, mannan. These observations suggest that prevention of the interaction of mycotin-related molecules on the surfaces of Mycobacteria with mannose-specific receptors on macrophages, offers an important approach for blocking attachment of pathogenic Mycobacteria to macrophages, thereby preventing infection.
致病性分枝杆菌会在宿主巨噬细胞中定殖。这些微生物附着于巨噬细胞是细菌侵入巨噬细胞之前的初步步骤。蛋白质印迹法证实，鸟分枝杆菌和结核分枝杆菌的细胞壁含有与肌动蛋白免疫相关的分子，肌动蛋白是耻垢分枝杆菌中发现的一种凝集素。我们已经证明，抗肌动蛋白抗体或肌动蛋白特异性糖甘露聚糖可显著抑制分枝杆菌与巨噬细胞的黏附。这些观察结果表明，阻止分枝杆菌表面与肌动蛋白相关的分子与巨噬细胞上的甘露糖特异性受体相互作用，为阻断致病性分枝杆菌与巨噬细胞的附着从而预防感染提供了一种重要方法。
