Elia G, Chinery R, Hanby A M, Poulsom R, Wright N A
RCS/ICRF Histopathology Unit, London, UK.
Histochem J. 1994 Aug;26(8):644-7. doi: 10.1007/BF00158289.
Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to detect its mRNA, due to the absence of a suitable antibody. The aim of the present study was to develop and characterize a monoclonal antibody against the human spasmolytic polypeptide, using a combination of immunohistochemistry and hybridization in situ. After immunoblotting, the antibody detected a 14 kDa protein in gastrointestinal tissue extracts from the stomach and small intestine only. Using immunohistochemistry, human spasmolytic polypeptide showed a distinctive staining pattern in the duodenum which co-localized with its mRNA. The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide.
人解痉多肽（hSP）是三叶肽家族中不断增加的一员，该家族在身体的离散区域表达，最显著的是胃肠道。由于缺乏合适的抗体，对解痉多肽定位的许多研究都依赖原位杂交来检测其mRNA。本研究的目的是结合免疫组织化学和原位杂交技术，开发并鉴定一种针对人解痉多肽的单克隆抗体。免疫印迹后，该抗体仅在胃和小肠的胃肠道组织提取物中检测到一种14 kDa的蛋白质。使用免疫组织化学方法，人解痉多肽在十二指肠呈现出独特的染色模式，且与mRNA共定位。免疫反应性肽与其mRNA的共定位提供了充分证据，表明该抗体确实识别了人解痉多肽。
