口蹄疫病毒两个可变翻译起始位点的功能分析
Functional analysis of the two alternative translation initiation sites of foot-and-mouth disease virus.
作者信息
Cao X, Bergmann I E, Füllkrug R, Beck E
机构信息
Department of Microbiology, State University at Stony Brook, New York 11794-8621.
出版信息
J Virol. 1995 Jan;69(1):560-3. doi: 10.1128/JVI.69.1.560-563.1995.
Abstract
The effect of deletion of each of the two authentic polyprotein translation initiation sites of foot-and-mouth disease virus on viral protein synthesis and replication was analyzed. Deletion of either the first or the second initiation site led to the expression of only one form of the leader protein, L or L', respectively, but in vitro processing of the viral polyprotein and cleavage of eIF-4 gamma were not affected by either deletion. Whereas RNA in which the first translation initiation site had been deleted led to the production of viruses in transfected BHK cells, deletion of the second translation initiation site abolished virus replication.
摘要
分析了口蹄疫病毒两个真实多聚蛋白翻译起始位点的缺失对病毒蛋白合成和复制的影响。删除第一个或第二个起始位点分别导致仅一种形式的前导蛋白L或L'的表达，但病毒多聚蛋白的体外加工和eIF-4γ的切割均不受任何一种缺失的影响。虽然删除了第一个翻译起始位点的RNA在转染的BHK细胞中导致了病毒的产生，但删除第二个翻译起始位点则消除了病毒复制。
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本文引用的文献
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