Compans R W, Melsen L R, de la Torre J C
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
Virus Res. 1994 Sep;33(3):261-8. doi: 10.1016/0168-1702(94)90107-4.
A line of Madin Darby canine kidney (MDCK) cells persistently infected with Borna disease virus was examined by electron microscopy. Thin sections revealed the presence of intracytoplasmic virus-like particles ranging from 50-100 nm in diameter. Nuclei of the infected cells exhibited accumulation of electron-dense granular structures 15-18 nm in diameter. The intracytoplasmic particles were roughly spherical with a limiting membrane, suggesting the presence of a lipid-containing envelope. The internal structure consisted of strand-like material which in some cases was condensed underneath the envelope. The possible relationship of these particles to Borna disease virions is discussed.
利用电子显微镜对持续感染博尔纳病病毒的犬肾传代细胞（MDCK）系进行了检查。超薄切片显示存在直径为50 - 100纳米的胞浆内病毒样颗粒。受感染细胞的细胞核呈现出直径为15 - 18纳米的电子致密颗粒状结构的聚集。胞浆内颗粒大致呈球形，有一层界膜，提示存在含脂质包膜。内部结构由条索状物质组成，在某些情况下，这些物质在包膜下方浓缩。讨论了这些颗粒与博尔纳病病毒粒子可能的关系。
