Kohno T, Goto T, Takasaki T, Morita C, Nakaya T, Ikuta K, Kurane I, Sano K, Nakai M
Department of Microbiology, Takatsuki, Osaka 569-8686, Japan.
J Virol. 1999 Jan;73(1):760-6. doi: 10.1128/JVI.73.1.760-766.1999.
Borna disease virus (BDV), a negative nonsegmented single-stranded RNA virus, has not been fully characterized morphologically. Here we present what is to our knowledge the first data on the fine ultrastructure and morphogenesis of BDV. The supernatant of MDCK cells persistently infected with BDV treated with n-butyrate contained many virus-like particles and more BDV-specific RNA than that of untreated samples. The particles were spherical, enveloped, and approximately 130 nm in diameter; had spikes 7 nm in length; and reacted with BDV p40 antibody. A thin nucleocapsid, 4 nm in width, was present peripherally in contrast to the thick nucleocapsid of hemagglutinating virus of Japan. The BDV particles reproduced by budding on the cell surface.
博尔纳病病毒（BDV）是一种负链非节段单链RNA病毒，其形态学特征尚未完全明确。在此，我们展示了据我们所知关于BDV精细超微结构和形态发生的首批数据。用正丁酸盐处理的持续感染BDV的MDCK细胞上清液中含有许多病毒样颗粒，且比未处理样品含有更多的BDV特异性RNA。这些颗粒呈球形，有包膜，直径约130纳米；有长度为7纳米的刺突；并与BDV p40抗体发生反应。与日本血凝病毒的厚核衣壳不同，宽度为4纳米的薄核衣壳存在于外周。BDV颗粒通过在细胞表面出芽进行复制。
