Schwarz S M, Heird W C
Department of Pediatrics, College of Physicians and Surgeons, Columbia University, NY.
Am J Clin Nutr. 1994 Dec;60(6):879-86. doi: 10.1093/ajcn/60.6.879.
To further define the effects of feeding on small intestinal ontogeny, naturally suckled and formula-fed beagle pups were studied over the first 120 h of life. In suckled animals, proximal jejunal and midintestinal mucosal weight and protein and DNA contents increased > 75% by 24 h (P < 0.005), with no further increases at 120 h. In formula-fed pups, no mucosal mass changes were found between 0 and 72 h of life. At 120 h, proximal jejunal mucosal weight and protein and DNA contents were significantly greater than in newborns (P < 0.005) and were similar to values for suckled animals at the same time. No significant mid-intestinal or terminal ileal growth was noted in formula-fed pups at any time. Specific and total activities of proximal jejunal brush border lactase, sucrase, and alkaline phosphatase were significantly greater in suckled vs formula-fed animals at 120 h. In a parallel study to assess postnatal effects of mature milk vs colostrum, significant mucosal growth at 24 h of life was demonstrated in pups suckled by surrogate dams who had whelped 21 d previously. These data indicate that both natural suckling (colostrum or milk) and formula feeding support enteric mucosal growth in newborn dogs; however, the two feeding regimens are characterized by unique ontogenic patterns of intestinal mucosal growth and function.
为了进一步明确喂养对小肠个体发育的影响，我们对自然哺乳和人工喂养的比格幼犬出生后的前120小时进行了研究。在自然哺乳的幼犬中，空肠近端和小肠中部黏膜重量、蛋白质和DNA含量在24小时内增加超过75%（P<0.005），在120小时时没有进一步增加。在人工喂养的幼犬中，出生后0至72小时内未发现黏膜质量变化。在120小时时，空肠近端黏膜重量、蛋白质和DNA含量显著高于新生幼犬（P<0.005），与同时期自然哺乳的幼犬相似。在任何时候，人工喂养的幼犬小肠中部或回肠末端均未观察到显著生长。在120小时时，自然哺乳的幼犬空肠近端刷状缘乳糖酶、蔗糖酶和碱性磷酸酶的比活性和总活性显著高于人工喂养的幼犬。在一项评估成熟乳与初乳对出生后影响的平行研究中，由21天前分娩的代孕母犬哺乳的幼犬在出生后24小时出现了显著的黏膜生长。这些数据表明，自然哺乳（初乳或乳汁）和人工喂养均能支持新生犬肠道黏膜生长；然而，这两种喂养方式具有独特的肠道黏膜生长和功能个体发育模式。
