Zhang H, Malo C, Boyle C R, Buddington R K
Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762-5759, USA.
J Nutr. 1998 Aug;128(8):1302-10. doi: 10.1093/jn/128.8.1302.
Structural and functional responses of the intestine to colostrum, milk replacer, oral electrolyte solution and food deprivation were examined during the first 6 h after birth in pigs. Total intestinal weight, surface area and mucosal mass were highest (P < 0.05) in pigs fed colostrum. The other diet groups did not differ, except that food-deprived pigs had lower surface area than the other groups. Feeding colostrum was associated with higher mucosal protein content (P < 0.05). Total intestinal brush border membrane protein content of pigs fed milk replacer, oral electrolyte solution and food-deprived pigs were 61, 44 and 56%, respectively, of those fed colostrum (P < 0.05). Pigs fed colostrum had higher total mucosal maltase activities than those that were food deprived, and total brush border membrane activities were higher than in those fed oral electrolyte solution. Total intestinal brush border membrane aminooligopeptidase activity was higher in pigs fed colostrum than in those given oral electrolyte solution or deprived of food, but total intestinal homogenate activities did not differ among groups. Diet influenced lactase activity only in the mid-region, and sucrase was not responsive to diet. Intestinal glucose transport capacity by intact intestinal tissues did not differ among diet groups. The ability of brush border membrane vesicles to actively accumulate glucose was lost when pigs were fed colostrum and milk replacer, but not when fed oral electrolyte solution or deprived of food. Our findings reveal how diet during the first 6 h after birth influences the structure and functional characteristics of the intestine. The responses vary between brush border membrane proteins and intestinal regions, and appear to differ from those described for older animals.
研究了出生后最初6小时内仔猪肠道对初乳、代乳品、口服电解质溶液和食物剥夺的结构和功能反应。喂食初乳的仔猪肠道总重量、表面积和黏膜质量最高（P<0.05）。其他饮食组之间没有差异，只是食物剥夺组的表面积低于其他组。喂食初乳与较高的黏膜蛋白含量相关（P<0.05）。喂食代乳品、口服电解质溶液的仔猪以及食物剥夺组仔猪的肠道刷状缘膜蛋白总含量分别为喂食初乳仔猪的61%、44%和56%（P<0.05）。喂食初乳的仔猪总黏膜麦芽糖酶活性高于食物剥夺组，总刷状缘膜活性高于喂食口服电解质溶液的仔猪。喂食初乳的仔猪肠道刷状缘膜氨肽酶总活性高于喂食口服电解质溶液或食物剥夺组的仔猪，但肠道匀浆总活性在各组间无差异。饮食仅影响肠道中部区域的乳糖酶活性，蔗糖酶对饮食无反应。完整肠道组织的肠道葡萄糖转运能力在不同饮食组间无差异。当仔猪喂食初乳和代乳品时，刷状缘膜囊泡主动积累葡萄糖的能力丧失，但喂食口服电解质溶液或食物剥夺时则未丧失。我们的研究结果揭示了出生后最初6小时内的饮食如何影响肠道的结构和功能特征。这些反应在刷状缘膜蛋白和肠道区域之间存在差异，并且似乎与年长动物的情况不同。
