Wälivaara B, Aronsson B O, Rodahl M, Lausmaa J, Tengvall P
Linköping University, Laboratory of Applied Physics, Sweden.
Biomaterials. 1994 Aug;15(10):827-34. doi: 10.1016/0142-9612(94)90038-8.
Adsorption of albumin (HSA) and fibrinogen (Fib) from human blood plasma onto titanium surfaces with varying oxide properties was studied with an enzyme-linked immunosorbent assay. The intrinsic activation of blood coagulation (contact activation) was studied in vitro using a kallikrein-sensitive substrate. The sample surfaces were characterized with Fourier transform Raman spectroscopy. Auger electron spectroscopy and atomic force microscopy. Low Fib and high HSA adsorption was observed for all titanium samples except for the radio frequency plasma-treated and water-incubated samples, which adsorbed significantly lower amounts of both. Oxide thickness and carbon contamination showed no influence on protein adsorption or contact activation. Smooth samples with a surface roughness (Rrms) < 1 nm showed some correlation between surface wettability and adsorption of Fib and HSA, whereas rough surfaces (Rrms > 5 nm) did not. To varying degrees, all titanium surfaces indicated activation of the intrinsic pathway of coagulation as determined by their kallikrein formation in plasma.
通过酶联免疫吸附测定法研究了人血浆中的白蛋白（HSA）和纤维蛋白原（Fib）在具有不同氧化物特性的钛表面上的吸附情况。使用激肽释放酶敏感底物在体外研究了血液凝固的内在激活（接触激活）。用傅里叶变换拉曼光谱、俄歇电子能谱和原子力显微镜对样品表面进行了表征。除了射频等离子体处理和水孵育的样品吸附的两种蛋白质数量明显较低外，所有钛样品均观察到低纤维蛋白原和高白蛋白吸附。氧化物厚度和碳污染对蛋白质吸附或接触激活没有影响。表面粗糙度（Rrms）<1nm的光滑样品在表面润湿性与纤维蛋白原和白蛋白吸附之间显示出一定的相关性，而粗糙表面（Rrms>5nm）则没有。不同程度上，所有钛表面都表明通过其在血浆中激肽释放酶的形成来激活凝血的内源性途径。
