Hayakawa Y, Yazaki K, Yamanaka A, Tanaka T
Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan.
Insect Mol Biol. 1994 May;3(2):97-103. doi: 10.1111/j.1365-2583.1994.tb00156.x.
DNA purified from polydnavirus particles isolated from the parasitoid wasp Cotesia kariyai contained double-stranded closed circular molecules which were polydisperse in molecular weight. 2 days after viral injection into the host armyworm larvae viral DNA was detected in all tested larval tissues, including haemocytes, fat body, nerve cord and brain. Viral transcripts were also observed in all the tissues of virus-injected larvae. The most specific tissue was haemocytes because more viral DNA and RNA was detected than in the other tissues. Viral transcripts were not detected in haemocytes of virus-injected larvae of common cutworm. The present data suggest that C. kariyai virus (CkV) gene expression occurs species-specifically, although viral DNA can be detected in all tested tissues of the habitual host armyworm.
从寄生蜂甘蓝夜蛾盘绒茧蜂分离得到的多分DNA病毒颗粒中纯化出的DNA含有双链闭环分子，其分子量呈多分散性。将病毒注射到宿主粘虫幼虫体内2天后，在所有测试的幼虫组织中都检测到了病毒DNA，包括血细胞、脂肪体、神经索和大脑。在注射病毒的幼虫的所有组织中也观察到了病毒转录本。最具特异性的组织是血细胞，因为在其中检测到的病毒DNA和RNA比其他组织更多。在注射病毒的小地老虎幼虫的血细胞中未检测到病毒转录本。目前的数据表明，甘蓝夜蛾盘绒茧蜂病毒（CkV）基因表达具有物种特异性，尽管在习惯性宿主粘虫的所有测试组织中都能检测到病毒DNA。
