Han J L, Yan W W, Qian Q J
Institute of Hematology, Hospital of Blood Diseases, Chinese Academy of Medical Sciences, Tianjin.
Zhonghua Yi Xue Za Zhi. 1994 Jul;74(7):424-7, 456.
We developed two leukemic cell lines (K562 HHT and L1210 HHT) stably 16.7 fold and 13.4 fold resistant to HHT respectively with which the culture were treated. Both cell lines were also cross-resistant to DOX, VCR, DNR and Mel. Increased expression of MDR1 gene in the both lines was noted. To further evaluate the implications of MDR1 in HHT resistance. We studied the expression of MDR1 in sensitive and HHT-resistant sublines of K562 by ABC with an monoclonal antibody against P170, JSB-1. K562 HHT cells were positive but sensitive cells were negative. Additionally, the increased drug resistance was associated with increased level of expression of alpha and pi class GST gene, but not with increased level of expression of mu class GST gene.
我们培育出了两种白血病细胞系（K562 HHT和L1210 HHT），它们对三尖杉酯碱（HHT）的耐药性分别稳定提高了16.7倍和13.4倍，并用其进行培养。这两种细胞系对阿霉素（DOX）、长春新碱（VCR）、柔红霉素（DNR）和马法兰（Mel）也具有交叉耐药性。发现这两种细胞系中多药耐药基因1（MDR1）的表达均增加。为了进一步评估MDR1在对HHT耐药中的作用，我们用抗P170单克隆抗体JSB-1通过免疫组织化学法（ABC）研究了K562敏感亚系和对HHT耐药亚系中MDR1的表达。K562 HHT细胞呈阳性，而敏感细胞呈阴性。此外，耐药性增加与α和π类谷胱甘肽S-转移酶（GST）基因表达水平升高有关，但与μ类GST基因表达水平升高无关。
