Waltzer L, Logeat F, Brou C, Israel A, Sergeant A, Manet E
Unité de Virologie Humaine, ENS-INSERM U412, Ecole Normale Supérieure de Lyon, France.
EMBO J. 1994 Dec 1;13(23):5633-8. doi: 10.1002/j.1460-2075.1994.tb06901.x.
The Epstein-Barr virus (EBV) protein EBNA2, which is essential for the immortalization of human primary B cells by EBV, acts as a transcriptional activator of cellular and viral genes. Specific responsive elements have been characterized in several of the promoters activated by EBNA2. They all share the core sequence GTGGGAA. EBNA2 does not, however, bind to these sequences directly, but appears to be targeted to them by a cellular protein. A similar core sequence has recently been identified as a high-affinity binding site for the human recombination signal sequence binding protein RBP-J kappa. Here we provide evidence that RBP-J kappa binds to specific sequences in EBNA2-responsive elements. Our results also demonstrate that RBP-J kappa makes direct physical contact with EBNA2 in solution and recruits EBNA2 to its cognate DNA sequences, suggesting that RBP-J kappa may mediate EBNA2 transactivation of both cellular and viral genes.
爱泼斯坦-巴尔病毒（EBV）蛋白EBNA2是EBV使人类原代B细胞永生化所必需的，它作为细胞和病毒基因的转录激活因子发挥作用。在几个由EBNA2激活的启动子中已经鉴定出了特定的反应元件。它们都共享核心序列GTGGGAA。然而，EBNA2并不直接与这些序列结合，而是似乎由一种细胞蛋白将其靶向到这些序列上。最近，一个类似的核心序列已被鉴定为人类重组信号序列结合蛋白RBP-Jκ的高亲和力结合位点。在这里，我们提供证据表明RBP-Jκ与EBNA2反应元件中的特定序列结合。我们的结果还表明，RBP-Jκ在溶液中与EBNA2直接发生物理接触，并将EBNA2招募到其同源DNA序列上，这表明RBP-Jκ可能介导EBNA2对细胞和病毒基因的反式激活作用。
