Cubel R C, Alferes A C, Cohen B J, Nascimento J P
Instituto Biomédico, Universidade Federal Fluminense, Niteró, Brazil.
J Clin Microbiol. 1994 Aug;32(8):1997-9. doi: 10.1128/jcm.32.8.1997-1999.1994.
Human parvovirus B19 recently was shown to agglutinate baboon and human erythrocytes. We have now demonstrated that both recombinant and native B19 antigens agglutinate rhesus, cynomolgus, and Saimiri monkey erythrocytes. Using cynomolgus erythrocytes and the recombinant antigen, we developed an immunoglobulin M (IgM) antibody capture hemadherence test (MACHAT) for the detection of specific B19 IgM antibodies in human sera. The results obtained with MACHAT were compared with those obtained with an IgM capture enzyme immunoassay (MACEIA) employing the native antigen routinely used in our laboratory. For 229 patient serum samples, we found 96% agreement between the results of the two assays. There was some evidence that MACHAT was slightly more sensitive than MACEIA. Our results add to the range of erythrocytes that can be agglutinated by B19 virus and show that native as well as recombinant antigens may be used in MACHAT.
人细小病毒B19最近被证明可凝集狒狒和人的红细胞。我们现已证实，重组和天然B19抗原均可凝集恒河猴、食蟹猴和松鼠猴的红细胞。利用食蟹猴红细胞和重组抗原，我们开发了一种免疫球蛋白M（IgM）抗体捕获血凝试验（MACHAT），用于检测人血清中的特异性B19 IgM抗体。将MACHAT获得的结果与使用我们实验室常规使用的天然抗原的IgM捕获酶免疫测定（MACEIA）获得的结果进行比较。对于229份患者血清样本，我们发现两种检测结果的一致性为96%。有证据表明MACHAT比MACEIA稍敏感。我们的结果增加了可被B19病毒凝集的红细胞种类，并表明天然抗原和重组抗原均可用于MACHAT。
