Cubel R C, Oliveira S A, Brown D W, Cohen B J, Nascimento J P
Departamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
J Clin Microbiol. 1996 Jan;34(1):205-7. doi: 10.1128/jcm.34.1.205-207.1996.
Serum and saliva samples were simultaneously collected from patients with B19 infection. Specimens were collected in a period of 1 to 18 days after the onset of symptoms. Saliva samples were collected with a commercial device, OraSure. The quality of these samples was evaluated by determining the concentration of total immunoglobulin G (IgG) by an enzyme immunoassay. The concentration of IgG in these samples ranged from 4.8 to > 250 mg/liter. B19 infection was confirmed for 20 patients by testing sera in a 1: 100 dilution by an IgM capture enzyme immunoassay (MACEIA) and an IgM capture hemadherence test (MACHAT). Saliva samples from these IgM-positive patients were tested neat by MACEIA and MACHAT. IgM could be detected in 11 of 20 (55%) samples by MACEIA and in 15 of 18 (83%) samples by MACHAT. Serum and saliva samples from a further 17 patients with rash were also tested. All of these specimens were unreactive by both assays. These results show that saliva may be a convenient alternative to serum for the diagnosis of recent B19 infection.
从B19感染患者中同时采集血清和唾液样本。样本在症状出现后的1至18天内采集。唾液样本用商业设备OraSure采集。通过酶免疫测定法测定总免疫球蛋白G（IgG）的浓度来评估这些样本的质量。这些样本中IgG的浓度范围为4.8至>250毫克/升。通过用IgM捕获酶免疫测定法（MACEIA）和IgM捕获血细胞吸附试验（MACHAT）对血清进行1:100稀释检测，确诊了20例患者感染B19。通过MACEIA和MACHAT对这些IgM阳性患者的唾液样本进行原液检测。通过MACEIA可在20份样本中的11份（55%）中检测到IgM，通过MACHAT可在18份样本中的15份（83%）中检测到IgM。还对另外17例有皮疹的患者的血清和唾液样本进行了检测。所有这些样本在两种检测中均无反应。这些结果表明，唾液可能是诊断近期B19感染的一种方便的替代血清的样本。
