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利用人O型红细胞和重组细小病毒B19抗原开发和评估捕获免疫球蛋白G和M血凝吸附试验

Development and evaluation of capture immunoglobulin G and M hemadherence assays by using human type O erythrocytes and recombinant parvovirus B19 antigen.

作者信息

Wang Q Y, Erdman D D

机构信息

Maternal and Child Health Hospital of Shaanxi Province, Xi'an, People's Republic of China.

出版信息

J Clin Microbiol. 1995 Sep;33(9):2466-7. doi: 10.1128/jcm.33.9.2466-2467.1995.

Abstract

The capacity of human parvovirus B19 to agglutinate human type O erythrocytes was used to develop immunoglobulin G and M antibody capture hemadherence assays. When results of these assays were compared with those of corresponding antibody capture enzyme immunoassays using a well-characterized panel of 125 serum specimens, a 96.8% overall agreement was obtained between the two methods.

摘要

利用人细小病毒B19凝集O型人红细胞的能力,开发了免疫球蛋白G和M抗体捕获血凝试验。当使用一组125份特征明确的血清标本,将这些试验的结果与相应的抗体捕获酶免疫试验的结果进行比较时,两种方法之间的总体一致性达到了96.8%。

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本文引用的文献

1
Antibody capture haemadherence tests for parvovirus B19-specific IgM and IgG.
J Virol Methods. 1993 Nov;45(1):27-37. doi: 10.1016/0166-0934(93)90137-g.
6
The use of labeled fusion protein for detection of B19 parvovirus IgM antibodies by an immunocapture test.
J Virol Methods. 1991 Apr;32(1):21-30. doi: 10.1016/0166-0934(91)90181-x.
7
Haemagglutination by parvovirus B19.细小病毒B19引起的血细胞凝集
J Gen Virol. 1992 Aug;73 ( Pt 8):2147-9. doi: 10.1099/0022-1317-73-8-2147.
8
Parvovirus B19 and human disease.
Adv Intern Med. 1992;37:431-55.

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