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利用人O型红细胞和重组细小病毒B19抗原开发和评估捕获免疫球蛋白G和M血凝吸附试验

Development and evaluation of capture immunoglobulin G and M hemadherence assays by using human type O erythrocytes and recombinant parvovirus B19 antigen.

作者信息

Wang Q Y, Erdman D D

机构信息

Maternal and Child Health Hospital of Shaanxi Province, Xi'an, People's Republic of China.

出版信息

J Clin Microbiol. 1995 Sep;33(9):2466-7. doi: 10.1128/jcm.33.9.2466-2467.1995.

DOI:10.1128/jcm.33.9.2466-2467.1995
PMID:7494048
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228440/
Abstract

The capacity of human parvovirus B19 to agglutinate human type O erythrocytes was used to develop immunoglobulin G and M antibody capture hemadherence assays. When results of these assays were compared with those of corresponding antibody capture enzyme immunoassays using a well-characterized panel of 125 serum specimens, a 96.8% overall agreement was obtained between the two methods.

摘要

利用人细小病毒B19凝集O型人红细胞的能力,开发了免疫球蛋白G和M抗体捕获血凝试验。当使用一组125份特征明确的血清标本,将这些试验的结果与相应的抗体捕获酶免疫试验的结果进行比较时,两种方法之间的总体一致性达到了96.8%。

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本文引用的文献

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Antibody capture haemadherence tests for parvovirus B19-specific IgM and IgG.
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Application to immunoglobulin M capture hemadherence assays of hemagglutination of monkey erythrocytes by native and recombinant human parvovirus B19 antigens.天然和重组人细小病毒B19抗原对猴红细胞进行血凝反应在免疫球蛋白M捕获血凝试验中的应用。
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Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测人细小病毒B19的抗体和抗原。
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Self-assembled B19 parvovirus capsids, produced in a baculovirus system, are antigenically and immunogenically similar to native virions.在杆状病毒系统中产生的自组装B19细小病毒衣壳在抗原性和免疫原性上与天然病毒粒子相似。
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Haemagglutination by parvovirus B19.细小病毒B19引起的血细胞凝集
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Parvovirus B19 and human disease.
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