来自牛脑的Rab鸟嘌呤核苷酸解离抑制剂（RabGDI）的结晶及初步晶体学数据。

Crystallization and preliminary crystallographic data for Rab guanine nucleotide dissociation inhibitor (RabGDI) from bovine brain.

作者信息

Schalk I J, Stura E A, Matteson J, Wilson I A, Balch W E

机构信息

Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037.

出版信息

J Mol Biol. 1994 Dec 9;244(4):469-73. doi: 10.1006/jmbi.1994.1744.

DOI:10.1006/jmbi.1994.1744

PMID:7990134

Abstract

X-ray quality crystals of Rab guanine nucleotide dissociation inhibitor (RabGDI) from bovine brain expressed in Escherichia coli have been obtained from 1.73 M ammonium sulfate. The crystals are prismatic long rods and belong to the monoclinic space group P21 with approximate cell dimensions a = 91.9 A, b = 43.5 A, c = 63.2 A, beta = 104.5 degrees and one molecule per asymmetric unit. The crystals are stable in the X-ray beam and diffract to at least 2.3 A. Reverse screening, streak seeding and macroseeding methods were used to obtain and improve the crystals.

摘要

已从1.73 M硫酸铵中获得在大肠杆菌中表达的来自牛脑的Rab鸟嘌呤核苷酸解离抑制剂（RabGDI）的X射线质量晶体。晶体为棱柱状长棒，属于单斜空间群P21，晶胞尺寸约为a = 91.9 Å，b = 43.5 Å，c = 63.2 Å，β = 104.5°，每个不对称单元有一个分子。这些晶体在X射线下稳定，衍射分辨率至少为2.3 Å。采用反向筛选、条纹接种和宏观接种方法来获得和改善晶体。