Wilson R J, Breckenridge L, Blackshaw S E, Connolly P, Dow J A, Curtis A S, Wilkinson C D
Department of Cell Biology, University of Glasgow, Scotland, UK.
J Neurosci Methods. 1994 Jul;53(1):101-10. doi: 10.1016/0165-0270(94)90150-3.
Planar extracellular electrode arrays provide a non-toxic, non-invasive method of making long-term, multisite recordings with moderately high spatial frequency (recording sites per unit area). This paper reports advances in the use of this approach to record from and stimulate single identified leech neurons in vitro. A modified enzyme treatment allowed identified neurons to be extracted with very long processes. Multisite extracellular recordings from the processes of such isolated neurons revealed both the velocity and direction of action potential propagation. Propagation in two cell types examined was from the broken stump towards the cell body (antidromic). This was true for spontaneous action potentials, action potentials produced by injecting current into the cell body and extracellular stimulation of the extracted process via a planar extracellular electrode. These results extend previous findings which have shown that the tip of the broken stump of extracted neurons has a high density of voltage-activated sodium channels. Moreover they demonstrate the applicability of extracellular electrode arrays for recording the electrical excitability of single cells.
平面细胞外电极阵列提供了一种无毒、非侵入性的方法，可用于以适度高的空间频率（每单位面积的记录位点）进行长期、多位点记录。本文报道了使用这种方法在体外对单个已识别的水蛭神经元进行记录和刺激方面取得的进展。一种改良的酶处理方法能够提取出具有很长轴突的已识别神经元。对这些分离神经元的轴突进行多位点细胞外记录，揭示了动作电位传播的速度和方向。在所研究的两种细胞类型中，动作电位都是从断端向细胞体传播（逆向传播）。自发动作电位、通过向细胞体注入电流产生的动作电位以及通过平面细胞外电极对提取的轴突进行细胞外刺激所产生的动作电位都是如此。这些结果扩展了先前的研究发现，即已提取神经元断端的尖端具有高密度的电压激活钠通道。此外，它们还证明了细胞外电极阵列在记录单个细胞电兴奋性方面的适用性。
