Rabini R A, Fumelli P, Galassi R, Dousset N, Taus M, Ferretti G, Mazzanti L, Curatola G, Solera M L, Valdiguié P
Division of Diabetology, Italian National Research Center on Aging, Ancona, Italy.
Metabolism. 1994 Dec;43(12):1470-4. doi: 10.1016/0026-0495(94)90003-5.
The aim of the present study was to determine if low-density lipoproteins (LDLs) and red blood cell (RBC) membranes from diabetic patients present an increased susceptibility to lipoperoxidation, which might be related to the increased incidence of atherosclerosis in diabetes. LDLs and RBC membranes were isolated from 11 insulin-dependent (IDDM) and 18 non-insulin-dependent diabetic (NIDDM) patients and exposed to a peroxidative stress by incubation with phenylhydrazine. The susceptibility to peroxidation was determined by measuring the production of thiobarbituric acid-reactive substances (TBARS) after the incubation. The following parameters were also evaluated: plasma glucose, triglycerides (TG), phospholipids (PL), total and high-density lipoprotein (HDL) cholesterol, apolipoprotein (apo) A-I, apo B, hemoglobin A1c (HbA1c), LDL PL and cholesterol, LDL fatty acid composition, and RBC membrane PL and cholesterol. Although they were apparently normolipidemic, diabetic patients showed an increased susceptibility to peroxidation in LDLs and erythrocyte membranes as compared with control subjects. The amount of arachidonic acid in LDLs and the PL concentration of RBC membranes from diabetic patients were significantly higher than in normal subjects. The increased lipoperoxidability of both RBC membranes and LDLs might play a central role in the pathogenesis of the vascular complications of diabetes mellitus.
本研究的目的是确定糖尿病患者的低密度脂蛋白（LDL）和红细胞（RBC）膜是否对脂质过氧化更敏感，这可能与糖尿病患者动脉粥样硬化发病率增加有关。从11名胰岛素依赖型（IDDM）和18名非胰岛素依赖型糖尿病（NIDDM）患者中分离出LDL和RBC膜，并通过与苯肼孵育使其暴露于过氧化应激。通过测量孵育后硫代巴比妥酸反应性物质（TBARS）的产生来确定对过氧化的敏感性。还评估了以下参数：血浆葡萄糖、甘油三酯（TG）、磷脂（PL）、总胆固醇和高密度脂蛋白（HDL）胆固醇、载脂蛋白（apo）A-I、apo B、糖化血红蛋白（HbA1c）、LDL PL和胆固醇、LDL脂肪酸组成以及RBC膜PL和胆固醇。尽管糖尿病患者明显血脂正常，但与对照组相比，他们的LDL和红细胞膜对过氧化更敏感。糖尿病患者LDL中花生四烯酸的含量和RBC膜的PL浓度显著高于正常受试者。RBC膜和LDL脂质过氧化能力的增加可能在糖尿病血管并发症的发病机制中起核心作用。
