Issa F, Gerhardt G A, Bartko J J, Suddath R L, Lynch M, Gamache P H, Freedman R, Wyatt R J, Kirch D G
Neuropsychiatry Branch, National Institute of Mental Health, Washington, DC 20032.
Psychiatry Res. 1994 Jun;52(3):237-49. doi: 10.1016/0165-1781(94)90069-8.
Recent hypotheses and findings indicate that measurements of interactions between cerebrospinal fluid (CSF) biogenic amine systems, rather than measurement of CSF biogenic amine metabolites, better correlate with clinically important findings in schizophrenia. To test hypotheses, we used a recent technological advance in high performance liquid chromatography with electrochemical detection and combined it with multivariate statistical analyses to study biogenic amine concentrations in CSF in schizophrenia. This approach enabled the study of the interactions of several metabolites of each of the three major neurotransmitter pathways (dopaminergic, noradrenergic, and serotonergic) to test existing hypotheses regarding the neurobiochemical basis of schizophrenia. Twenty biogenic amines, their metabolites, and other compounds from 24 medication-free schizophrenic patients and 12 normal control subjects were simultaneously measured using a recently developed technique of gradient high performance liquid chromatography coupled with a 16-channel electrochemical array detector. After covariation for storage time, results of a stepwise discriminant function analysis comparing the control and patient groups identified tryptophan, tryptophol, and epinephrine as discriminating variables. Hotelling's paired T2 test from a subgroup of schizophrenic patients studied while they were and were not receiving neuroleptic treatment did not yield any significant differences between subgroups. A discussion of the findings and a comparison with previous studies of CSF biogenic amines in schizophrenia are presented.
近期的假说和研究结果表明，测量脑脊液（CSF）生物胺系统之间的相互作用，而非测量CSF生物胺代谢产物，与精神分裂症的临床重要发现具有更好的相关性。为了验证假说，我们采用了高效液相色谱与电化学检测的一项最新技术进展，并将其与多变量统计分析相结合，以研究精神分裂症患者脑脊液中的生物胺浓度。这种方法能够研究三种主要神经递质途径（多巴胺能、去甲肾上腺素能和5-羟色胺能）中每种途径的几种代谢产物之间的相互作用，从而验证关于精神分裂症神经生化基础的现有假说。使用最近开发的梯度高效液相色谱技术与16通道电化学阵列检测器，同时测量了24名未用药的精神分裂症患者和12名正常对照受试者的20种生物胺、它们的代谢产物以及其他化合物。在对储存时间进行协变量调整后，比较对照组和患者组的逐步判别函数分析结果确定色氨酸、色醇和肾上腺素为判别变量。对一组精神分裂症患者在接受和未接受抗精神病药物治疗时进行研究，采用霍特林配对T2检验，结果显示亚组之间没有任何显著差异。本文对研究结果进行了讨论，并与先前关于精神分裂症患者脑脊液生物胺的研究进行了比较。
