Kaufman P L
Exp Eye Res. 1987 Feb;44(2):283-91. doi: 10.1016/s0014-4835(87)80012-x.
Living cynomolgus monkeys received intracameral infusions of pilocarpine HCI (P)1 mg ml-1, followed by P + cytochalasin B (CB) 50 microgram ml-1; total outflow facility, measured by two-level constant pressure perfusion, tripled after P alone and then remained stable when CB was added. Other eyes received CB 25 micrograms ml-1 or 50 micrograms ml-1, followed by CB + P1 mg ml-1; facility quadrupled to sextupled after CB, but tended to decrease from those levels, although it remained above baseline, after P was added. The facility decrease accompanying the addition of P after CB might have related to the longer CB exposure, rather than to a specific P effect. These data indicate that despite different mechanisms of action and primary attack points on the outflow apparatus, near- or supramaximal P and CB doses are not additive, and are indeed mutually pre-emptive, in their facility increasing effect. These findings have implications in terms of trabecular-meshwork biophysics, fluid mechanics, and their pharmacological manipulation.
对成年食蟹猴进行前房内注射1毫克/毫升的盐酸毛果芸香碱（P），随后注射50微克/毫升的P + 细胞松弛素B（CB）；通过两级恒压灌注测量总房水流出率，单独注射P后总房水流出率增加两倍，添加CB后保持稳定。其他眼睛先注射25微克/毫升或50微克/毫升的CB，随后注射1毫克/毫升的CB + P；注射CB后房水流出率增加四倍至六倍，但添加P后，房水流出率虽仍高于基线水平，但有从这些水平下降的趋势。CB后添加P伴随的房水流出率下降可能与CB暴露时间较长有关，而非与P的特定作用有关。这些数据表明，尽管对房水流出装置的作用机制和主要攻击点不同，但接近或超过最大剂量的P和CB在增加房水流出率的作用方面并非相加，实际上是相互抢占的。这些发现对小梁网生物物理学、流体力学及其药理学操作具有启示意义。
