Ghosh C, Zhou Y L, Collodi P
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
Cell Biol Toxicol. 1994 Jun;10(3):167-76. doi: 10.1007/BF00757560.
ZF-L cells were derived from normal adult zebrafish liver, and have been growing in culture for more than 100 generations. The cells were derived in basal nutrient medium supplemented with fetal bovine serum (FBS), trout serum, trout embryo extract, bovine insulin and mouse epidermal growth factor. After 50 generations in culture, optimal growth of the cells was achieved in medium supplemented with FBS (5%) and trout serum (0.5%). ZF-L cells were hypodiploid (modal chromosome number = 46) and exhibited an epithelial morphology. ZF-L cell homogenates exhibited alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. The cells synthesized and released several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG.
ZF-L细胞源自正常成年斑马鱼肝脏，已在培养中生长超过100代。这些细胞在添加有胎牛血清（FBS）、鲑鱼血清、鲑鱼胚胎提取物、牛胰岛素和小鼠表皮生长因子的基础营养培养基中获得。培养50代后，在添加5% FBS和0.5%鲑鱼血清的培养基中实现了细胞的最佳生长。ZF-L细胞为亚二倍体（众数染色体数=46），呈现上皮形态。ZF-L细胞匀浆表现出丙氨酸和天冬氨酸转氨酶、葡萄糖-6-磷酸酶和碱性磷酸酶的酶活性。这些细胞合成并向培养基中释放了几种蛋白质，包括一种可被抗牛血清白蛋白IgG识别的70 kDa蛋白质。
