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Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos.

作者信息

Ghosh C, Collodi P

机构信息

Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907.

出版信息

Cytotechnology. 1994;14(1):21-6. doi: 10.1007/BF00772192.

DOI:10.1007/BF00772192
PMID:7765109
Abstract

The zebrafish has become a popular model for studies of vertebrate development and toxicology. However, in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum.

摘要

相似文献

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Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos.
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2
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4
Basic fibroblast growth factor stimulates proliferation and suppresses melanogenesis in cell cultures derived from early zebrafish embryos.碱性成纤维细胞生长因子刺激源自早期斑马鱼胚胎的细胞培养物增殖并抑制黑色素生成。
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Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8-cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human leukemia inhibitory factor.来自猪4细胞和8细胞胚胎以及在有或无蛋白质和人白血病抑制因子情况下体外培养的分离卵裂球的囊胚中内细胞团与滋养层细胞的比例。
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[Disordered mitoses and extranuclear chromatin in the cells of normally developing embryos of Brachydanio rerio].
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