Thorisdottir A S, Carias L L, Marshall S H, Green M, Zervos M J, Giorgio C, Mermel L A, Boyce J M, Medeiros A A, Fraimow H
Medical Research Service, Department of Veterans Affairs Medical Center, Cleveland, Ohio.
J Infect Dis. 1994 Dec;170(6):1539-48. doi: 10.1093/infdis/170.6.1539.
Enterococci expressing resistance to antimicrobial agents are increasingly important nosocomial pathogens. Effective strategies to prevent or abort outbreaks of resistant enterococcal infection will rely on an accurate understanding of the mechanisms by which these organisms spread. A 1065-bp insertion-like sequence (IS6770) is present in varying copy numbers in > 90% of enterococcal strains thus far examined. Hybridization patterns resulting from hybridization of enterococcal genomic DNA with an internal IS6770 probe vary considerably between unrelated strains and correlate well with results of pulsed-field gel electrophoresis and field-inversion gel electrophoresis in identifying clonal relationships among enterococcal isolates. IS6770 analysis of several outbreaks of resistant enterococci has confirmed the spread of single resistant clones rather than the emergence of resistance within the resident flora. These results suggest that IS6770 hybridization will be a useful tool for tracing the epidemiology of nosocomial enterococcal infections.
对抗菌药物具有耐药性的肠球菌日益成为重要的医院病原体。预防或终止耐药性肠球菌感染暴发的有效策略将依赖于准确了解这些微生物传播的机制。在迄今为止检测的90%以上的肠球菌菌株中，存在一个1065 bp的插入样序列（IS6770），其拷贝数各不相同。肠球菌基因组DNA与IS6770内部探针杂交产生的杂交模式在不相关菌株之间差异很大，并且在鉴定肠球菌分离株之间的克隆关系方面与脉冲场凝胶电泳和场反转凝胶电泳的结果密切相关。对几起耐药性肠球菌暴发的IS6770分析证实了单一耐药克隆的传播，而不是在固有菌群中出现耐药性。这些结果表明，IS6770杂交将成为追踪医院内肠球菌感染流行病学的有用工具。
