IS18的特性研究，IS18是一种能够通过转座激活不动杆菌属13株BM2716沉默的aac(6')-Ij基因的元件。
Characterization of IS18, an element capable of activating the silent aac(6')-Ij gene of Acinetobacter sp. 13 strain BM2716 by transposition.
作者信息
Rudant E, Courvalin P, Lambert T
机构信息
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France.
出版信息
Antimicrob Agents Chemother. 1998 Oct;42(10):2759-61. doi: 10.1128/AAC.42.10.2759.
Abstract
Insertion sequence IS18 was detected by analysis of the spontaneous aminoglycoside resistant mutant Acinetobacter sp. 13 strain BM2716-1. Insertion of the element upstream from the silent acetyltransferase gene aac(6')-Ij created a hybrid promoter that putatively accounts for the expression of the aminoglycoside resistance gene. The 1, 074-bp IS18 element contained partially matched (20 out of 26 bases) terminal inverted repeats, one of which overlapped the 3' end of a 935-bp open reading frame potentially encoding a protein related to the transposases of the IS30 family. IS18 was found in 6 out of 29 strains of Acinetobacter sp. 13 but not in 10 strains each of A. baumannii and A. haemolyticus.
摘要
通过对自发产生氨基糖苷抗性的不动杆菌属13株BM2716 - 1突变体的分析，检测到插入序列IS18。该元件插入沉默乙酰转移酶基因aac(6')-Ij上游，产生了一个杂合启动子，推测该启动子导致了氨基糖苷抗性基因的表达。1074 bp的IS18元件包含部分匹配的（26个碱基中有20个）末端反向重复序列，其中一个与一个935 bp开放阅读框的3'端重叠，该开放阅读框可能编码一种与IS30家族转座酶相关的蛋白质。在29株不动杆菌属13菌株中有6株发现了IS18，但在鲍曼不动杆菌和溶血不动杆菌各10株菌株中未发现。
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